Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells.
Identifieur interne : 002673 ( Main/Exploration ); précédent : 002672; suivant : 002674Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells.
Auteurs : RBID : pubmed:17096094English descriptors
- KwdEn :
- Animals, Cell Proliferation (drug effects), Cell Proliferation (radiation effects), Cell Survival (drug effects), Cell Survival (radiation effects), Disease Models, Animal, Female, Heart Ventricles (drug effects), Heart Ventricles (radiation effects), Hematopoietic Stem Cells (drug effects), Hematopoietic Stem Cells (radiation effects), Hematopoietic Stem Cells (radionuclide imaging), Indium Radioisotopes (diagnostic use), Lung (drug effects), Lung (radiation effects), Mice, Mice, Inbred C57BL, Organometallic Compounds (diagnostic use), Oxyquinoline (analogs & derivatives), Oxyquinoline (diagnostic use), Radionuclide Imaging (methods), Radiopharmaceuticals (diagnostic use), Time Factors.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , diagnostic use : Indium Radioisotopes, Organometallic Compounds, Oxyquinoline, Radiopharmaceuticals.
- drug effects : Cell Proliferation, Cell Survival, Heart Ventricles, Hematopoietic Stem Cells, Lung.
- methods : Radionuclide Imaging.
- radiation effects : Cell Proliferation, Cell Survival, Heart Ventricles, Hematopoietic Stem Cells, Lung.
- radionuclide imaging : Hematopoietic Stem Cells.
- Animals, Disease Models, Animal, Female, Mice, Mice, Inbred C57BL, Time Factors.
Abstract
Cell-based therapy by transplantation of progenitor cells has emerged as a promising development for organ repair, but non-invasive imaging approaches are required to monitor the fate of transplanted cells. Radioactive labelling with (111)In-oxine has been used in preclinical trials. This study aimed to validate (111)In-oxine labelling and subsequent in vivo and ex vivo detection of haematopoietic progenitor cells.
DOI: 10.1007/s00259-006-0275-3
PubMed: 17096094
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells.</title><author><name sortKey="Nowak, Bernd" uniqKey="Nowak B">Bernd Nowak</name><affiliation wicri:level="3"><nlm:affiliation>Department of Nuclear Medicine, University Hospital, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany. be.nowak@arcor.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Nuclear Medicine, University Hospital, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Aix-la-Chapelle</settlement></placeName></affiliation></author><author><name sortKey="Weber, Christian" uniqKey="Weber C">Christian Weber</name></author><author><name sortKey="Schober, Andreas" uniqKey="Schober A">Andreas Schober</name></author><author><name sortKey="Zeiffer, Ute" uniqKey="Zeiffer U">Ute Zeiffer</name></author><author><name sortKey="Liehn, Elisa A" uniqKey="Liehn E">Elisa A Liehn</name></author><author><name sortKey="Von Hundelshausen, Philipp" uniqKey="Von Hundelshausen P">Philipp von Hundelshausen</name></author><author><name sortKey="Reinartz, Patrick" uniqKey="Reinartz P">Patrick Reinartz</name></author><author><name sortKey="Schaefer, Wolfgang M" uniqKey="Schaefer W">Wolfgang M Schaefer</name></author><author><name sortKey="Buell, Ulrich" uniqKey="Buell U">Ulrich Buell</name></author></titleStmt><publicationStmt><date when="2007">2007</date><idno type="doi">10.1007/s00259-006-0275-3</idno><idno type="RBID">pubmed:17096094</idno><idno type="pmid">17096094</idno><idno type="wicri:Area/Main/Corpus">002995</idno><idno type="wicri:Area/Main/Curation">002995</idno><idno type="wicri:Area/Main/Exploration">002673</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Proliferation (drug effects)</term><term>Cell Proliferation (radiation effects)</term><term>Cell Survival (drug effects)</term><term>Cell Survival (radiation effects)</term><term>Disease Models, Animal</term><term>Female</term><term>Heart Ventricles (drug effects)</term><term>Heart Ventricles (radiation effects)</term><term>Hematopoietic Stem Cells (drug effects)</term><term>Hematopoietic Stem Cells (radiation effects)</term><term>Hematopoietic Stem Cells (radionuclide imaging)</term><term>Indium Radioisotopes (diagnostic use)</term><term>Lung (drug effects)</term><term>Lung (radiation effects)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Organometallic Compounds (diagnostic use)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Oxyquinoline (diagnostic use)</term><term>Radionuclide Imaging (methods)</term><term>Radiopharmaceuticals (diagnostic use)</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Indium Radioisotopes</term><term>Organometallic Compounds</term><term>Oxyquinoline</term><term>Radiopharmaceuticals</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term><term>Cell Survival</term><term>Heart Ventricles</term><term>Hematopoietic Stem Cells</term><term>Lung</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Radionuclide Imaging</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Cell Proliferation</term><term>Cell Survival</term><term>Heart Ventricles</term><term>Hematopoietic Stem Cells</term><term>Lung</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Hematopoietic Stem Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Female</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cell-based therapy by transplantation of progenitor cells has emerged as a promising development for organ repair, but non-invasive imaging approaches are required to monitor the fate of transplanted cells. Radioactive labelling with (111)In-oxine has been used in preclinical trials. This study aimed to validate (111)In-oxine labelling and subsequent in vivo and ex vivo detection of haematopoietic progenitor cells.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">17096094</PMID><DateCreated><Year>2007</Year><Month>04</Month><Day>26</Day></DateCreated><DateCompleted><Year>2007</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1619-7070</ISSN><JournalIssue CitedMedium="Print"><Volume>34</Volume><Issue>5</Issue><PubDate><Year>2007</Year><Month>May</Month></PubDate></JournalIssue><Title>European journal of nuclear medicine and molecular imaging</Title><ISOAbbreviation>Eur. J. Nucl. Med. Mol. Imaging</ISOAbbreviation></Journal><ArticleTitle>Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells.</ArticleTitle><Pagination><MedlinePgn>715-21</MedlinePgn></Pagination><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">Cell-based therapy by transplantation of progenitor cells has emerged as a promising development for organ repair, but non-invasive imaging approaches are required to monitor the fate of transplanted cells. Radioactive labelling with (111)In-oxine has been used in preclinical trials. This study aimed to validate (111)In-oxine labelling and subsequent in vivo and ex vivo detection of haematopoietic progenitor cells.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Murine haematopoietic progenitor cells (10(6), FDCPmix) were labelled with 0.1 MBq (low dose) or 1.0 MBq (high dose) (111)In-oxine and compared with unlabelled controls. Cellular retention of (111)In, viability and proliferation were determined up to 48 h after labelling. Labelled cells were injected into the cavity of the left or right cardiac ventricle in mice. Scintigraphic images were acquired 24 h later. Organ samples were harvested to determine the tissue-specific activity.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Labelling efficiency was 75 +/- 14%. Cellular retention of incorporated (111)In after 48 h was 18 +/- 4%. Percentage viability after 48 h was 90 +/- 1% (control), 58 +/- 7% (low dose) and 48 +/- 8% (high dose) (p<0.0001). Numbers of viable cells after 48 h (normalised to 0 h) were 249 +/- 51% (control), 42 +/- 8% (low dose) and 32 +/- 5% (high dose) (p<0.0001). Cells accumulated in the spleen (86.6 +/- 27.0% ID/g), bone marrow (59.1 +/- 16.1% ID/g) and liver (30.3 +/- 9.5% ID/g) after left ventricular injection, whereas most of the cells were detected in the lungs (42.4 +/- 21.8% ID/g) after right ventricular injection.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Radiolabelling of haematopoietic progenitor cells with (111)In-oxine is feasible, with high labelling efficiency but restricted stability. The integrity of labelled cells is significantly affected, with substantially reduced viability and proliferation and limited migration after systemic transfusion.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nowak</LastName><ForeName>Bernd</ForeName><Initials>B</Initials><Affiliation>Department of Nuclear Medicine, University Hospital, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany. be.nowak@arcor.de</Affiliation></Author><Author ValidYN="Y"><LastName>Weber</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Schober</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Zeiffer</LastName><ForeName>Ute</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Liehn</LastName><ForeName>Elisa A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>von Hundelshausen</LastName><ForeName>Philipp</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Reinartz</LastName><ForeName>Patrick</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Schaefer</LastName><ForeName>Wolfgang M</ForeName><Initials>WM</Initials></Author><Author ValidYN="Y"><LastName>Buell</LastName><ForeName>Ulrich</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Eur J Nucl Med Mol Imaging</MedlineTA><NlmUniqueID>101140988</NlmUniqueID><ISSNLinking>1619-7070</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Radiopharmaceuticals</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Proliferation</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName><QualifierName MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName><QualifierName MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Heart Ventricles</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName><QualifierName MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hematopoietic Stem Cells</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName><QualifierName MajorTopicYN="N">radiation effects</QualifierName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lung</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName><QualifierName MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radionuclide Imaging</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radiopharmaceuticals</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>6</Month><Day>6</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>8</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2006</Year><Month>11</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>11</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>9</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>11</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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